Sunday, February 17, 2013

100 RI fire victims remembered in outdoor ceremony

A unidentified woman wipes her face as she reacts during ceremonies held to unveil plans for a permanent memorial on the site of The Station nightclub fire, Sunday, Feb. 17, 2013, in West Warwick, R.I. The 2003 blaze, which broke out when pyrotechnics for the rock band Great White ignited flammable packing foam that had been installed inside the club as soundproofing, took the lives of 100 people. (AP Photo/Steven Senne)

A unidentified woman wipes her face as she reacts during ceremonies held to unveil plans for a permanent memorial on the site of The Station nightclub fire, Sunday, Feb. 17, 2013, in West Warwick, R.I. The 2003 blaze, which broke out when pyrotechnics for the rock band Great White ignited flammable packing foam that had been installed inside the club as soundproofing, took the lives of 100 people. (AP Photo/Steven Senne)

Tonda Daniels, of South Kingstown, R.I., front, places an ornament on a makeshift memorial to her fallen sister Lori Durante at the site of the Station nightclub fire, Sunday, Feb. 17, 2013, in West Warwick, R.I. The Station Fire Memorial Foundation unveiled final plans to build a permanent memorial at the site during ceremonies Sunday. The 2003 blaze took the lives of 100 people. (AP Photo/Steven Senne)

Jason Zubee, left, and his wife Robin Zubee, right, both of North Kingstown, R.I., stand together Sunday, Feb. 17, 2013, in West Warwick, R.I., near makeshift memorials on the site of The Station nightclub fire. Zubee lost her cousin William Christopher Bonardi III in the 2003 blaze at the nightclub that killed 100 people. The Station Fire Memorial Foundation unveiled final plans to build a permanent memorial at the site during ceremonies Sunday. (AP Photo/Steven Senne)

Diane Gomes, of Johnston, R.I., left, and Elaine Grant, of Lincoln, R.I., center, help display artist's renderings of a planned permanent memorial for victims of The Station nightclub fire during ceremonies at the site of the fire, in West Warwick, R.I., Sunday, Feb. 17, 2013. The 2003 blaze, which broke out when pyrotechnics for the rock band Great White ignited flammable packing foam that had been installed inside the club as soundproofing, took the lives of 100 people. (AP Photo/Steven Senne)

Burn survivors of The Station nightclub fire Deb Wagner, of West Warwick, R.I., left, and Linda Fisher, of Chepachet, R.I., center, support one another as former R.I. Gov. Donald Carcieri, right, looks down during ceremonies on the site of the fire, Sunday, Feb. 17, 2013, in West Warwick. The Station Fire Memorial Foundation unveiled final plans to build a permanent memorial at the site during ceremonies Sunday. The 2003 blaze took the lives of 100 people. (AP Photo/Steven Senne)

(AP) ? Survivors of a 2003 nightclub fire that killed 100 people and relatives of those killed huddled together in bitter cold Sunday at the site of the blaze to mark the 10th anniversary of it.

Some brought flowers and paid their respects at the handmade crosses that dot the site for each person who died. Others cried and spoke of missing their loved ones and the difficulty of moving past such trauma.

"People that weren't here really don't understand why we can't let this stuff go. I was 30 seconds away from dying," said Walter Castle Jr., 39, a survivor who suffered third-degree burns in his lungs, throat and bronchial tubes. He said he lost many friends and was in counseling until 2009. Recently, as the 10th anniversary approached, he began having terrible nightmares and had to go back into counseling.

"It's just very tough," he said.

The anniversary of the blaze is Wednesday. The fire broke out when pyrotechnics for the rock band Great White ignited flammable packing foam that had been installed in the club as soundproofing. Last month, a fire at a nightclub in Brazil killed more than 230 people under circumstances that were eerily similar: A band's pyrotechnic display set fire to soundproofing foam.

Among those who spoke Sunday was former Gov. Don Carcieri, who took office the month before the fire and still gets choked up when speaking about it. He remembered the days families waited at a hotel for word that their loved ones' remains had been identified, and the anger everyone felt, asking how the tragedy could have happened. But he also remembered how people in Rhode Island, a state with a population of just 1 million, pulled together to help each other.

"At a time of our state's worst tragedy, in some sense, it was our people's finest hour," he said.

Angela Bogart, who was 19 when her mother, Jude Henault, was killed in the fire, said she has come to know and understand her mother more in the 10 years since she died, especially since she has become a mother herself.

"My mom lives in me in everything I do. I hear her voice wherever I go," she said. "When I walk hand-in-hand with my little girl, my mother is holding her other hand."

The ceremony also featured musical performances, a reading of the names of the people who died and 100 seconds of silence.

While somber, the annual gathering at the fire site took on a more hopeful tone this year than in years past because a foundation set up to build a permanent memorial secured ownership of the site in September after years of trying. On Sunday, the Station Fire Memorial Foundation released final plans for the memorial.

They call for a 30-foot-high entrance gate topped by an Aeolian harp. Wind passing through the harp will create music, a reminder that it was music that brought people together that night.

The permanent memorial will include an individual memorial for each person who died and commemorate the survivors, first responders and those who helped care for families of the dead and survivors in the weeks and months after the fire. It will also include a pavilion as a gathering place.

Families are being asked to remove the crosses and other personal mementos that have been left at the site at the makeshift memorial that has developed over the years. The items left behind will be buried in a capsule under an area that is now the parking lot. There will be no digging on the land under where the club once stood because of the fear of disturbing human remains.

While many of the materials and labor to build the memorial will be donated, foundation officials say they need to raise $1 million to $2 million to build and maintain it.

The foundation hopes to break ground in the spring. Construction of the memorial could take one to two years.

Gina Russo, who was badly burned in the fire and whose fianc? was killed, is president of the foundation and said the memorial would turn the site into something beautiful.

"It's a happy moment going forward," she said.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/386c25518f464186bf7a2ac026580ce7/Article_2013-02-17-RI%20Nightclub%20Fire/id-94c5094ebae143a69e0f0d751b3337f0

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Saturday, February 16, 2013

A microbial biorefinery provides new insight into how bacteria regulate genes

Friday, February 15, 2013

Microorganisms that can break down plant biomass into the precursors of biodiesel or other commodity chemicals might one day be used to produce alternatives to petroleum. But the potential of this "biorefinery" technology is limited by the fact that most microorganisms cannot break down lignin, a highly stable polymer that makes up as much as a third of plant biomass.

Streptomyces bacteria are among few microorganisms known to degrade and consume lignin. Now a group of researchers at Brown University has unlocked the genetic and molecular mechanisms behind a key part of that process. The results are published in the journal Nucleic Acids Research.

Jason Sello, professor of chemistry at Brown, and Rebecca Page, professor in biology in the Department of Molecular Biology, Cell Biology, and Biochemistry, directed the research with graduate students Jennifer Davis and Breann Brown.

"Aside from the implications for biotechnology, this work is significant because it yielded fundamental insights into how bacteria control the expression of their genes," Sello said. "Understanding how genes underlying lignin degradation are regulated could have practical importance in that we could possibly use this information to engineer bacteria that can convert this important component of plant biomass into the biofuels of high-value chemicals."

The consumption of lignin by Streptomyces bacteria is a multistep process. First, the bacteria release enzymes that depolymerize the lignin ? break it down into its constituent compounds. The bacteria take up the resulting compounds and use the carbon to support their growth and reproduction. Some of that lignin-derived carbon is converted into triglycerides, the building blocks of biodiesel, and other high-value compounds.

This latest research deals with the second part of that process, the ability of Streptomyces bacteria to metabolize aromatic compounds derived from lignin, which are highly stable and hard to break down.

In previous work, Sello and Davis identified a cluster of genes in Streptomyces bacteria that encode enzymes for breaking down a lignin-derived compound called protocatechuate. Under normal circumstances, those genes were inactive ? essentially switched off. Only when bacteria were grown in a medium containing protocatechuate did the genes switch on and produce the appropriate enzymes. In an effort to understand this phenomenon, Sello and Davis discovered that a transcription factor ? a kind of protein that attaches itself to DNA ? called PcaV was involved in switching the genes on and off. The next step, and the focus of this new research, was figuring out how PcaV controls gene expression.

Sello and Davis proposed a model for how it might work. They proposed that PcaV probably binds to DNA in a way that physically prevents the transcription of the lignin-degrading genes, turning them off. In order to explain how the genes are switched on in the presence of protocatechuate, they proposed that the compound might compromise the ability of PcaV to bind to DNA, which would expose the genes and allow them to be expressed.

A series of experiments provided support for their model. In a test tube, the researchers established that PcaV tightly binds to specific DNA sequences in close proximity to the gene cluster in question, validating the first half of the model. To confirm the second half, Sello and his colleagues exposed the PcaV-DNA complex to protocatechuate. They found that PcaV loses its affinity for DNA in the presence of the compound.

"So we can say that protocatechuate attenuates the DNA binding activity of the PcaV protein, which permits expression of the genes," Sello said. "We now have evidence that validates our model."

Sello and his colleagues then dug down into the process a little further. Using a technique called protein crystallography, the team examined the structure of PcaV in complex with protocatechuate, to gain insight into how the compound affects the capacity of the protein to bind DNA. They found that arginine-15, an amino acid in PcaV, is critical for binding both to protocatechuate and to DNA. On this basis, the group proposed that arginine-15 acts as a molecular switch that is flipped by protocatechuate.

"In the absence of protocatechuate, arginine 15 is important in the binding of PcaV to DNA in a manner that represses transcription," Sello said. "But when you introduce protocatechuate, the arginine spatially reorients to bind the compound. The accompanying conformational changes compromise the ability of the protein to bind to DNA."

An important protein

Beyond possible implications for bioenergy and sustainable chemistry, Sello says the work is meaningful for basic research in bacteriology. PcaV is a member of the MarR family of transcription factors, a family of 12,000 proteins that are known to regulate genes that influence virulence and drug resistance in other types of bacteria.

"It's a very important family of transcription factors in bacteria," Sello said. "Our paper is particularly important because it sheds light on how these proteins control gene expression and mediate responses to small molecules. While many studies of MarR family transcription factors have been published, ours is unique in the way that it synergistically integrates experimental methods from genetics, biochemistry, biophysics, and structural biology."

The research was funded by the National Science Foundation, the National Institutes of Health, and through a seed award from the Office of the Vice President of Research at Brown.

"The collegiality and size of the scientific community at Brown helps to make these collaborative, interdisciplinary research projects work," Sello said.

###

Brown University: http://www.brown.edu/Administration/News_Bureau

Thanks to Brown University for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

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Source: http://www.labspaces.net/126844/A_microbial_biorefinery_provides_new_insight_into_how_bacteria_regulate_genes

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How To Overcome The Economy Objection - Video ... - Sales Training

Despite the fact that our economy is now growing and we are finally moving away from the dreaded ?recession objection?, many sales people are finding that they are still coming up against the economy objection during their sales interactions and are struggling to close the deal because of it. So how do you overcome the economy objection and close more sales? Watch our short video to find out.

Happy Selling!

Sean McPheat

Managing Director

MTD Sales Training

www.mtdsalestraining.com

Have you downloaded my latest report ?The Sales Person?s Crisis?? Over 10,000 sales pros have. Click on the image below to find out why your very existence as a sales person is in doubt?

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Sean McPheat

Sean McPheat is a bestselling author and MD of international training firm MTD Sales Training, who have delivered training, coaching and consultancy to over 2,500 different organisations and over 50,000 staff from 23 different countries. Sean is regarded as a thought leader on modern day selling and business improvement, and has been featured on CNN, ITV, BBC, SKY, Forbes, Arena Magazine with over 250 other media credits to his name. Sean?s Sales Blog is visited by 5,000 people every week and over 60,000 managers and sales professionals across the world receive Sean's weekly email tips.


Source: http://www.mtdsalestraining.com/mtdblog/how-to-overcome-the-economy-objection-video-blog.html

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Friday, February 15, 2013

Build Your Own Emergency Phone Charger with Common Household Objects

Build Your Own Emergency Phone Charger with Common Household ObjectsBuild Your Own Emergency Phone Charger with Common Household Objects You never know when you're going to get stuck without a way to charge your cellphone (or batteries, lights, or anything else), but if you need to create a charging system in a pinch, YouTuber The King of Random shows off a simple trick to make a human powered charger using common household items.

The system uses a cordless drill, some yarn, aluminum foil, a mixer beater, a wooden fork, a two-by-four, and tape to create a hand cranked generator. Basically, you're tapping the charger into where the cordless drill would usually connect to a battery, and then sending that energy to the phone when you crank the drill. It's similar in theory to a hand-cranked charger we've featured before, but doesn't require any soldering or real know-how to make. Of course, with anything like this you're risking overheating or damaging the equipment, but it's a solution that works in a pinch.

Make an Emergency Phone Charger - MacGyver Style! | YouTube via Instructables

Source: http://feeds.gawker.com/~r/lifehacker/full/~3/GlF9lasD3VE/build-a-your-own-emergency-phone-charger-with-common-household-objects

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Kris Humphries? Lawyer Bails On Him In Divorce Case

Kris Humphries’ Lawyer Bails On Him In Divorce Case

Kris Humphries still wants annulmentKris Humphries’ lawyer, Marshall Waller, has filed paperwork to withdraw from the Kris Humphries and Kim Kardashian divorce case that has dragged on for over a year. The news comes just a day before he was set to meet with Kim’s attorney, Laura Wasser, for a court hearing. Waller cites an “irremediable breakdown of the ...

Kris Humphries’ Lawyer Bails On Him In Divorce Case Stupid Celebrities Gossip Stupid Celebrities Gossip News

Source: http://stupidcelebrities.net/2013/02/kris-humphries-lawyer-bails-on-him-in-divorce-case/

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